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Cdc50 has a direct interaction with Crm1 and inhibits its expression (Cao et al., 2019)

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A431 and RPE cells were lysed with 1% NP-40 lysis buffer containing 50 mM Tris-HCl at pH 7.5, 150 mM NaCl, 1 mM EGTA, 5 mM ATP, 5 mM MgCl 2 and complete protease inhibitor cocktail ( Roche ), homogenised using a 25-g needle and clarified by centrifugation at 20,000 g for 15 min at 4 C

doi: 10.1210/jc.2011-2407
