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Shaking the vial can physically break these chains apart (a process called shearing), destroying the molecules structure and rendering it ineffective for research

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Sanitize Everything: Once again, wipe the stoppers of both the testosterone vial and the reconstituted HCG vial with a fresh alcohol swab

The protein:ligand complex was crystallized after 3 days of incubation at 20 C in 20 mM zinc chloride and 20% (w/v) PEG 3,350 by the hanging-drop vapor diffusion method (2 L protein-ligand complex:2 L crystallization solution)