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From experimental data of kinetic measurements of BRET, following vehicle and baseline corrections, quantification of induced responses from individual peptide concentrations was determined by calculating the net area under curve from the 10 min response (AUC 0-10min) ) in GraphPad Prism version 9.0.1 (GraphPad Software Inc., San Diego, CA)

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For older researchers, regenerative protocols may need to be more comprehensive and longer in duration to compensate for these age-related deficits

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Which one is relevant depends on the pathway a given research question targets