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Briefly, frozen sections were treated with basic ethanol (80% ethanol/1% NaOH) for 5 min, and washed in 70% EtOH and ultrapure water for 2 min, respectively

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These compounds are synthesized via a common precursor pathway known as the phenylacrylic acid metabolic pathway, hence they are referred to as phenylpropanoids in studies of plant secondary metabolism (Liu et al., 2020)

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