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Protein samples were mixed with Laemmli sample buffer, boiled at 95C for 5 minutes, and separated on a 12% SDS-polyacrylamide gel

Cells were harvested by centrifugation at 4,400 g at 4 C for 15 min, and the medium was discarded

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[DOI] [PubMed] [Google Scholar] Wasserman GA, Liu X, Parvez F, Ahsan H, Factor-Litvak P, van Geen A, Slavkovich V, LoIacono NJ, Cheng Z, Hussain I, Momotaj H, Graziano JH