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Description
The cell lysates were quantified using Pierce 660 nm Protein Assay Reagent (Thermo Fisher Scientific, 22660) and Ionic Detergent Compatibility Reagent for Pierce 660 nm Protein Assay Reagent (Thermo Fisher Scientific, 22663)

[DOI] [PubMed] [Google Scholar] Le C

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A vast majority of our B12 intake comes from animal sources such as meat, fish, milk, cheese and eggs

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Based on the visual colour and appearance, spinach was commercially unacceptable after those storage times (shelf-life values)
