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Chromatographic conditions employ a C18 reversed-phase column, with acetonitrilewater solution (adjusted to an appropriate acidity with phosphoric acid) as the mobile phase

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These tests may require specialist laboratory referral Holotranscobalamin (active B12) where available, may be used as a second-line test when standard B12 results are borderline Intrinsic factor antibodies to identify pernicious anaemia, the most common cause in the UK Parietal cell antibodies may be useful if intrinsic factor antibodies are negative but pernicious anaemia is still suspected Thyroid function and folate levels to exclude other causes of similar symptoms According to current UK guidance, patients presenting with neurological symptoms suggestive of B12 deficiency should not have treatment delayed whilst awaiting test results

Key Takeaways Understanding the prevalence and impact of anemia is key to good care

References to obesity, adipose tissue, mitochondrial function, or glucose-related pathways on this page are provided for laboratory research context only

When you have time, read through Coeliac UKs info about diagnosis which is more detailed than the info I've given