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Description
The assembled DNA cassette was digested with SfiI and ligated upstream of C H 1-p3 into the SfiI-digested phagemid using T4 DNA ligase, yielding phagemids encoding Fabs linked to the N-terminus of a fragment of protein III (pIII: amino acids 230406) of filamentous phage M13 42
Binding specificity was demonstrated by the inability of Munc13-4 to pull down other STXs or to bind to the fusion regulator Vti1b (ref
doi: 10.1021/acsami.7b18927
Free Radical Biology and Medicine 95: 2742
Association between Serum Magnesium and Blood Lipids: Influence of Type 2 Diabetes and Central Obesity
A second mutation (G482A), which is also associated with this disorder, introduces a Pst1 site with decreased enzyme capacity [45]
