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Total RNA from the liver tissue (100 mg) and HepG2 cells were extracted using TRIzol reagent (#15596026, Invitrogen, Waltham, MA, USA) according to a previously described method [20]
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2 2.8.3 Widely targeted metabolomics analysis Frozen tissue specimens were homogenized and extracted using ice-cold methanol:water (7:3, v/v) supplemented with isotope-labeled internal standards,followed by multi-platform LC-MS/MS analysis
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It has been suggested that tricyclic antidepressants exert some of their therapeutic effect by inhibiting MAO [185]
