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Protein extracts were then subjected to SDSPAGE and transferred to Immobilin-FL polyvinylidene fluoride transfer membrane

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After 180 min of anaerobic incubation at 37 C, the entire culture volume was collected into 50 mL conical tubes with pipetting and scraping to capture adherent cells, and the cells were centrifuged at 4000 g for 10 min, yielding pellets with a wet weight of ~100 mg

We calibrate and validate the model with experimental data from control C3H mice and from the rd1 mouse model for the disease RP 37