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Highlights: RT-AM is cell permeable and can generate RT upon intracellular esterase cleaveage Easily quantitate GSH levels in live cells using confocal microscopy/FACS Signals from blue and green channels allow for deduction of GSH concentration, given appropriate calibration curve For calibration curve, see: RealThiol (RT) Glutathione (GSH) Calibration Probe Subcellular detection and localization of GSH is important in understanding the modulation of redox status, the effect of drugs, and the mechanisms of detoxification

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Photo by Snizhana Galytska/iStock/Getty Images Plus Vitamin B12, also known as cobalamin, is a water-soluble vitamin that is essential for neurologic function, intestinal function, red blood cell synthesis, and fatty acid and amino acid metabolism