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The His 6 -tag was cleaved off by PreScission protease and the resulting authentic 306 amino acid M pro (see Figure S1A in supplemental material) and C300S M pro were further purified by SEC using a HiLoad Superdex 200 pg column (GE Healthcare) in 20 mM Tris, pH 7.5, 150 mM NaCl, and 2 mM DTT

Ipratropium bromide: a review of its pharmacological properties and therapeutic efficacy in asthma and chronic bronchitis

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PhAN9 (A12M): PhAN9 _A12M_fw, PhAN9 _A12M_rv, PhAN9 (C13S): PhAN9 _C13S_fw, PhAN9 _C13S_rv, PhAN9 (N108H): PhAN9 _N108H_fw, PhAN9 _N108H_rv (Supplementary Table 4) using pANT15 as template