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To enhance the signal, sections were incubated in ABC solution (VectorLabs, catalog #PK-6100) for 1 h followed by a DAB solution (DAB, Sigma-Aldrich, catalog #91-95-2, 0.05%) of cobalt chloride (0.05%) and nickel ammonium sulfate (0.05%) to produce a black precipitate in c- fos + cells

This grants them direct access to the DNA within the cell nucleus

& Stephenson, L