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In support of this observation, over expression of GS in a yeast model failed to increase GSH levels, whereas overexpression of GCL did [18]
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The scFvs demonstrate superior tumor penetration and faster pharmacokinetics compared to full-length antibodies owing to their small size and absence of the Fc domain 11
Next, the coverslips were washed 2 10 min in Duolink Wash Buffer B (0.2 M Tris-Base, 0.1 M NaCl, pH 7.5) and then again in 1 mL of Duolink Wash Buffer B diluted at 1:100 in 1X PBS for 1 min
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