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Nonspecific binding was a potential problem with the purification of his-tagged proteins from various expression systems, especially from the lower expressed recombinant his-tagged proteins or higher expressed proteins interacting with other cellular proteins

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By upregulating the expression of the lipase ABHD16A, tumor cells release lysophosphatidylserine, which acts on GPR34 to downregulate the expression of antitumor effectors in type 1 innate lymphoid cells [150]

Collectively, the gutbrain, erythrocytebrain, and kidneybrain axes highlight the systemic complexity of PD pathogenesis, where peripheral dysfunction contributes to central neurodegeneration through diverse molecular and cellular pathways