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The effect of semaglutide subcutaneous injection at 2.4 mg per week in patients with HFpEF and obesity (BMI 30 kg/m 2 ) but without diabetes was tested in STEP-HFpEF [29]

To investigate active metabolism hECs were co-incubated with metabolic inhibitor sodium azide (1 mg/ml) and nanocarriers at 37 C for 4 h

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Hence, the most appropriate antagonist would be a compound that exhibits inhibitory activity in the low micromolar range, thus preserving the physiological function of the receptors while inhibiting them when overactivated

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Immunoblotting Following the indicated treatment, cell lysates were generated in ice cold RIPA lysis buffer (20 mM Tris-HCl (VWR, 97061-258), pH 7.5, 150 mM NaCl (Fisher Scientific, S271), 1 mM EGTA, 1 mM EDTA [Sigma-Aldrich, E5134], 1% sodium deoxycholate [Sigma-Aldrich, D6750], 1% NP-40 [Sigma-Aldrich, 74385]) supplemented with protease inhibitors (Fisher Scientific, PIA32955)