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An equivalent amount of protein from each animal was mixed with 4 sample buffer with or without 2-mercaptoethanol, then separated by SDSpolyacrylamide gel electrophoresis, and transferred electrophoretically to a PVDF membrane (Merck Millipore)

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So, not only is it necessary to not mix your whey protein into a hot beverage or food, you must also consider that you should not follow consuming your whey protein with drinking a hot beverage such as hot coffee or tea, or eating a hot meal

Yang, X., Liu, X., Wang, L., Xu, J

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