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Cell culture Swiss 3T3 fibroblasts, obtained from American Type Culture Collection (ATCC CCL 92), were maintained at 37 in Dulbecco's modified Eagle's medium supplemented with 25 mM HEPES (pH 7.4), 10% (v/v) fetal bovine serum, 100 units/mL penicillin, and 100 mg/mL streptomycin

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Both cytosolic and peroxisomal isoforms of Cu/Zn-SOD rely on zinc for stability and activity, and MTs serve as zinc buffers, releasing zinc under oxidative stress to fine-tune redox signaling and enzyme expression 94

In another study using a murine model of multiple food allergies, FAHF-2 also protected against allergen-induced anaphylaxis in multiple food allergies (peanut, fish, and egg) [180, 185]

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