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A fuse-silica glass plate (thickness, 1.5 mm) was placed in the optical path for the LO signal, allowing phase modulation for the LO signal

The goal is to avoid contaminating either vial and to ensure accurate dosing

Taking a second dose creates an overdose scenario with significantly amplified side effects including severe nausea and GI distress

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Next, to assess whether this early motor deficit was also rescued by the downregulation of Gsto2 , additional cohorts of mutants were injected at 3 months with Gsto2 -shRNA or scrambled shRNA and tested 3 weeks later for unsupported rearing