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5 Type 2 diabetes and the role of insulin signaling in PD Type 2 diabetes (T2DM) and PD are both age-associated diseases that have a high prevalence due to increased life expectancy

Samples were divided into pre- and post-VDZ treatment and responders (R, responding at week 52, n = 9) or non-responders (NR, not responding at week 52)

After microscopic examination, oocytes at metaphase II stages (MII, with the polar body extrusion) were collected and cultured in 500 l of fertilization droplets as SOF medium supplemented with 5% fetal bovine serum, 2 mM sodium pyruvate, 10 g/ml heparin sodium and 1 mM caffeine in a CO 2 incubator (38.5C, 90%N 2 , 5%CO 2 and 5%O 2 ) (32)

Since the Standard GSSG is packed under reduced pressure, open after adding 0.5 % SSA solution with a syringe through a rubber septum

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