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A proposal for validation of antibodies

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Immunology 128:e206-221

Molecular mechanisms Two major pathophysiological causes of many diseases are endothelial dysfunction and oxidative stress

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Measurement of gastric myeloperoxidase activity After the homogenization step with 50 mM potassium phosphate buffer, myeloperoxidase (MPO) activity in the gastric tissues was measured based on H2O2-dependent oxidation of o-dianisidine.2HCl using a spectrophotometer (PG Instruments Ltd., UK) at 460 nm wavelength