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Each mouse was given 120 seconds to find an escape hole and tested continuously for 4 trials with the escape time for each trial recorded
This happens in our eggs, too

In this review, we describe recent discoveries providing evidence for the contribution of the GHGHRIGF1 axis on mitochondrial biogenesis, mitophagy (or autophagy), and mitochondrial function under multiple physiological conditions
Eisbruch, A, Rhodus N, Rosenthal D, Murphy B, Rasch C, Sonis S, Scarantino C, Brizel D

There is also a third pathway that makes nasal delivery uniquely interesting for certain applications

doi: 10.1038/nrendo.2011.183
