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platensis strain LB 2340 from the Texas Algal Culture collection UTEX was grown under constant white light using an alkaline Spirulina medium: solution I (162 mM NaHCO 3 , 38 mM Na 2 CO 3 , and 2.9 mM K 2 HPO 4 in 500 ml dH 2 O) and II (29.4 mM NaNO 3 , 5.74 mM K 2 SO 4 , 17.1 mM NaCl, 0.81 mM MgSO 4 , 0.27 mM CaCl 2 in 500 ml dH 2 O) were autoclaved separately, combined after cooling, and 2 ml of a sterile-filtered 0.1 mM vitamin B 12 solution was added

Kim, Y.G

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The authors suggest that CuHIS treatment likely enhanced copper transport via residual CTR1 activity, or perhaps permeated cells via a CTR1-independent process resulting in the observed modest clinical improvement (Batzios et al., 2022)

10.1039/c1ob05996c 17 BorhadeS

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