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In comparison, rhGPx3 mutant seems more likely to catalyze the reaction just like GPx4, which is the only monomeric member among the GPx family, due to the fact that the mutant exists as a monomer in its native state

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The following assays are routinely used for purity analysis: Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) - a denaturing gel that separates proteins based on size