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Briefly, the lyophilized internal standards were reconstituted according to the manufacturers specifications, and the daily working solution was prepared by diluting the reconstituted internal standards 1:100 (v/v) using the extraction solution

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Essential role of NOXA1 in generation of reactive oxygen species induced by oxidized low-density lipoprotein in human vascular endothelial cells

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Am J Kidney Dis 41(Suppl4):S4S12 Acknowledgements We acknowledge the contribution of the co-researchers

Sigma-Aldrich) on ice for 30 min, then at 37C for 5 min, rinsed in 2% BSA in PBS, and dissociated with gentle pipetting