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The increase in Alpha-counts is proportional to the mono- or poly-ubiquitination of the GST-tagged p21

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Negative reactions are rare, as this procedure is both safe and well-proven to be beneficial

IGF-I acts predominantly via the IGF-I receptor (IGF-IR), a transmembrane protein consisting of two extracellular -subunits, which contain the IGF-I binding site, and two transmembrane -subunits that have a cluster of three tyrosine residues, which undergo phosphorylation and activation upon IGF-I binding to mediate canonical signaling pathways necessary for cell survival and growth

Ten g of total RNA at a concentration greater than 1 g/ml from each sample was used for microarray hybridization