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Description
THP-1 cells were differentiated into macrophages by 100 nM of phorbol-12-myristate-13-acetate (Sigma-Aldrich), and differentiated into M1 by adding LPS (100 ng/mL, Sigma-Aldrich) and IFN- (20 ng/mL, R&D Systems) for 24 h, and M2 cells with M-CSF (20 ng/mL, Peprotech), IL-4 (40 ng/mL, R&D Systems), and IL-13 (40 ng/mL, R&D Systems) for 4 days

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On the vertical axis, we have whats known as probability density, which we will return to in in a moment
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Communication between the zinc and tetrahydrobiopterin binding sites in nitric oxide synthase

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