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2.5 Determination of protein concentration The concentration of purified proteins was determined using Bradford reagent (Sigma-Aldrich) according to the manufacturers instructions with bovine serum albumin as a standard

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Current research shows that androgen regulation in PCOS has been the most extensively studied, with various treatment mechanisms identified, including the upregulation of LDHA, HK2, and PKM2 expression, as well as the modulation of pathways such as IRE1-XBP1 and ERK

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The signaling pathway of necroptosis has been largely elucidated [207]

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