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Cerebrospinal fluid (CSF) was collected with a cell count of 6/mm3, normal protein (33.9 mg/dL), and normal glucose (66.32 mg/dL)

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Research has confirmed that the Nle4 and D-Phe7 substitutions in MT-1 produce substantially extended in vitro and in vivo stability relative to native -MSH , documenting resistance to endopeptidase cleavage at the position 7 Phe bond and prevention of methionine oxidation that inactivates native -MSH, resulting in prolonged receptor occupancy and sustained downstream signalling