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Pol J Environ Stud 28(2):515528
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Proteins were precipitated with 80% methanol for 30 minutes at -20C and removed by centrifugation at 20000xg for 10 minutes
We do know, however, that CF patients have a diminished immune response that is attributed to decreased GSH levels leading to premature apoptosis in macrophages and neutrophils recruited to the lung [87]
