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The use of a single compound also provides unequivocal evidence of its efficacy, free from the confounding effects of other substances

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Trichloroethylene metabolism in the rat ovary reduces oocyte fertilizability
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In fact, it can create DNA strand breaks caused by the formation of NO-dependent reactive species such as peroxynitrite, which directly attack the DNA and cause failure to correct single-strand breaks, leading to the formation of double-strand breaks, which are probably responsible for cell death