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After incubation, 1 mL of absolute EtOH was added and SP3 beads were magnetically captured and washed three times with 80% EtOH, then resuspended in 200 L 50 mM HEPES buffer (pH 7.4) containing 1 mM TCEP and incubated for 3 min at RT

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Lee JY, Vyas CK, Kim GG, Choi PS, Hur MG, Yang SD, Kong YB, Lee EJ, Park JH

After LysoPrime Green and pHLys Red intensity values were obtained, lysosomal pH of Lyso-low and Lyso-high GBM cell lines was calculated by pHLys Red/ LysoPrime Green values based on the generated standard curve