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The membranes were blocked with 5% bovine serum albumin (BSA) or 5% dry skimmed milk in Tris-buffered saline containing 0.05% (v/v) Tween-20 (TBST) for 2 h and incubated with the appropriate primary antibody in blocking buffer for 2 h at room temperature

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Broad cytoprotective signaling, including ERK1/2 and FAKpaxillin activation, which enhance cell survival, adhesion, and motility at injury sites

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The concentrations of immunoglobulins G (IgG), M [IgM], and A [IgA] were detected in plasma samples using available kits (Catalog No.: MBS260043, MBS706158 and MBS564152, respectively) provided by Mybiosource.com (San Diego, CA, USA)