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in submerged batch process in shake flask culture

PLC protein has the biggest size among all PLC family members - 230 kDa and consists of several functional domains including core domain (consisting of EF, X and Y subdomains) which possesses an ability to hydrolyze PIP2, pleckstrin-homology PH and C2 domains, GTP-exchanging (CDC25-like) domain at the C-terminus and two Ras-associating domains at N-terminus [4-6]