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Needles are designed for one-time use and become microscopically dull even after a single puncture through skin or a rubber stopper

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Both use the same 5-days-on/2-days-off schedule, making them naturally synchronized

Theres no universally best time, but most users inject: Once daily in the morning , or Split into two doses : AM and PM for more stable coverage (especially for injury or gut repair) For injury-specific cases, timing the dose post-workout or before sleep may support natural repair cycles

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[DOI] [PMC free article] [PubMed] [Google Scholar] Sikiric P, Jadrijevic S, Seiwerth S, Sosa T, et al