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An equivalent amount of protein from each animal was mixed with 4 sample buffer with or without 2-mercaptoethanol, then separated by SDSpolyacrylamide gel electrophoresis, and transferred electrophoretically to a PVDF membrane (Merck Millipore)

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[DOI] [PubMed] [Google Scholar] [104].Stocker S, Maurer M, Ruppert T, Dick TP, A role for 2-Cys peroxiredoxins in facilitating cytosolic protein thiol oxidation, Nat

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