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Barcode plasmid pool preparation To generate the bacterial CloneSelect barcode library for the Zeocin resistance marker, a semi-random oligonucleotide pool KI#405 encoding 5-ATGCCGVNNVNNVNNVNNVNNTAA-3 was chemically synthesized (Supplementary Table 2)

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Its purpose is gastrointestinal adaptation, not clinical effect

Emphasize the importance of regular follow-up visits and laboratory tests to evaluate the effectiveness of oral antidiabetic agents and make necessary adjustments

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