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the protein was concentrated (50 kD cutoff: Millipore, Sigma-Aldrich) and loaded on a S200 Superdex Increase 10/300 column (Cytiva) equilibrated in G-buffer LCP
The PNS1-encoding plasmid was transformed into the S
Reconstitute: Add 3.0 mL bacteriostatic water 3.33 mg/mL concentration
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