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Interestingly, when we checked for the presence of WIPI2 in the MYTHO-immunoprecipitated complex, we showed a reduction in WIPI2 binding to MYTHO when motif 3 (M3) and 4 (M4) were altered, and a slight reduction with mutated M1 (Figure 7B and Supplemental Figure 10C)

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a decrease in both the liver weight and hepatic triglyceride content in experimental animals was observed, thereby reconfirming the antibody's significant inhibitory effect on hepatic fat accumulation